Human C21orf63 is a Heparin-binding Protein

Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative 'galactose-binding lectin domains'. By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulp...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2009-09, Vol.146 (3), p.369-373
Main Authors: Mitsunaga, Kanae, Harada-Itadani, Jun, Shikanai, Toshihide, Tateno, Hiroaki, Ikehara, Yuzuru, Hirabayashi, Jun, Narimatsu, Hisashi, Angata, Takashi
Format: Article
Language:English
Subjects:
C21orf63
Cloning, Molecular
Databases, Protein
Epithelial Cells - metabolism
Fluorescent Antibody Technique
Galectins
Gastric Mucosa - metabolism
Gene Expression
glycan microarray
Glycosaminoglycans - metabolism
heparan sulphate
heparin
Heparin - analogs & derivatives
Heparin - metabolism
Heparitin Sulfate - metabolism
Humans
Immobilized Proteins - metabolism
lectins
Lectins - chemistry
Lectins - immunology
Lectins - metabolism
Membrane Proteins - chemistry
Membrane Proteins - immunology
Membrane Proteins - metabolism
Microarray Analysis
Microscopy, Fluorescence
Mutant Proteins - chemistry
Mutant Proteins - immunology
Mutant Proteins - metabolism
Organ Specificity
Protein Binding
Protein Structure, Tertiary
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - metabolism
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Summary:Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative 'galactose-binding lectin domains'. By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulphate. The C-terminal galactose-binding lectin domain of C21orf63 is necessary for heparin binding. The inability of other human proteins with galactose-binding lectin domains to interact with heparin suggests that heparin binding is a unique property of C21orf63. Results of real-time polymerase chain reaction and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues.
ISSN:0021-924X
1756-2651
DOI:10.1093/jb/mvp079