Isolation and sequence analysis of a β-tubulin gene from arbuscular mycorrhizal fungi

A full-length β-tubulin gene has been cloned and sequenced from Gigaspora gigantea and Glomus clarum, two arbuscular mycorrhizal fungi (AMF) species in the phylum Glomeromyota. The gene in both species is organized into five exons and four introns. Both genes are 94.9% similar and encode a 447 amino...

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Bibliographic Details
Published in:Mycorrhiza 2009-09, Vol.19 (7), p.501-513
Main Authors: Msiska, Zola, Morton, Joseph B
Format: Article
Language:English
Subjects:
Agriculture
Amino Acid Sequence
Base Sequence
Biomedical and Life Sciences
Ecology
Forestry
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungi - chemistry
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Life Sciences
Microbiology
Molecular Sequence Data
Mycorrhizae - chemistry
Mycorrhizae - classification
Mycorrhizae - genetics
Mycorrhizae - isolation & purification
Original Paper
Phylogeny
Plant Sciences
Sequence Analysis, DNA
Tubulin - chemistry
Tubulin - genetics
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Summary:A full-length β-tubulin gene has been cloned and sequenced from Gigaspora gigantea and Glomus clarum, two arbuscular mycorrhizal fungi (AMF) species in the phylum Glomeromyota. The gene in both species is organized into five exons and four introns. Both genes are 94.9% similar and encode a 447 amino acid protein. In comparison with other fungal groups, the amino acid sequence is most similar to that of fungi in the Chytridiomycota. The codon usage of the gene in both AMF species is broad and biased in favor of an A or a T in the third position. The four introns varied in length from 87 to 168 bp for G. gigantea and from 90 to 136 bp for G. clarum. Of all fungi in which full-length sequences have been published, only AMF do not have an intron before codon 174. The introns positioned at codons 174 and 257 in AMF match the position of different introns in β-tubulin genes of some Zygomycete, Basidiomycete, and Ascomycete fungi. The 5' and 3' splice site consensus sequences are similar to those found in introns of most fungi. Sequence analysis from single-strand conformation polymorphism analysis confirmed the presence of two β-tubulin gene copies in G. clarum, but only one copy was evident in G. gigantea based on Southern hybridization analysis.
ISSN:0940-6360
1432-1890
DOI:10.1007/s00572-009-0248-z