High-Performance Thin-Layer Chromatographic Determination of Rabeprazole Sodium and Domperidone in Combined Dosage Form

A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. T...

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Bibliographic Details
Published in:Journal of AOAC International 2009-07, Vol.92 (4), p.1064-1067
Main Authors: SANTOSH VILASHCHAND GANDHI, KHAN, Samina I, JADHAV, Rashmi T, JADHAV, Saket S, JADHAV, Gaurav A
Format: Article
Language:English
Subjects:
2-Pyridinylmethylsulfinylbenzimidazoles - analysis
Antagonists (Biochemistry)
Antiemetics - analysis
Biological and medical sciences
Calibration
Chemical properties
Chemistry, Pharmaceutical
Chromatography, Thin Layer
Domperidone - analysis
Drug Combinations
Food industries
Fundamental and applied biological sciences. Psychology
Identification and classification
Indicators and Reagents
Methods
Proton Pump Inhibitors - analysis
Rabeprazole
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
Tablets
Thin layer chromatography
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Summary:A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. The retention factors for RAB and DOM were found to be 0.53 +/- 0.12 and 0.32 +/- 0.20. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 50-800 ng/band for both RAB and DOM. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
ISSN:1060-3271
1944-7922