Toxoplasma gondii : P30 peptides recognition pattern in human toxoplasmosis

In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients...

Full description

Saved in:
Bibliographic Details
Published in:Experimental parasitology 2009-10, Vol.123 (2), p.199-202
Main Authors: Cardona, Nestor, de-la-Torre, Alejandra, Siachoque, Heber, Patarroyo, Manuel A., Gomez-Marin, Jorge E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis of Variance
Animals
Antigens, Protozoan - chemistry
Antigens, Protozoan - immunology
Antigens, Surface - chemistry
Antigens, Surface - immunology
Biological and medical sciences
Chronic Disease
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Human antibodies
Humans
Immune Sera - immunology
Immunoglobulin G - immunology
Infant
Life cycle. Host-agent relationship. Pathogenesis
Molecular Sequence Data
Peptides
Peptides - chemistry
Peptides - immunology
Protozoa
Protozoan Proteins - chemistry
Protozoan Proteins - immunology
SAG1
Toxoplasma - chemistry
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis - immunology
Toxoplasmosis, Congenital - immunology
Toxoplasmosis, Ocular - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more strongly peptides from the protein’s carboxy-terminus, being peptide 2017 (amino acids 301–320) the one most strongly recognized by sera from patients with ocular toxoplasmosis. Serum samples collected from 13 patients without ocular infection, 13 with inactive chorioretinal scars, 6 with active ocular infection and 10 seronegative individuals were then screened for anti-2017 IgG. Peptide 2017 was recognized by all patients’ samples but not by sera from T. gondii-seronegative individuals. No statistically significant differences were found between the absorbance levels of groups with and without lesions or with active or inactive ocular lesions, as determined by ANOVA.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2009.06.017