Functional role of aquaglyceroporin 7 expression in the pancreatic beta-cell line BRIN-BD11

Both mouse and rat pancreatic islet β‐cells were recently found to express aquaglyceroporin 7 (AQP7). In the present study, the expression and role of AQP7 in the function of BRIN‐BD11 cells were investigated. AQP7 mRNA and protein were detected by RT‐PCR and Western blot analysis, respectively. In...

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Published in:Journal of cellular physiology 2009-11, Vol.221 (2), p.424-429
Main Authors: Delporte, C., Virreira, M., Crutzen, R., Louchami, K., Sener, A., Malaisse, W.J., Beauwens, R.
Format: Article
Language:English
Subjects:
Animals
Aquaporins - genetics
Aquaporins - metabolism
Cell Line
Gene Expression Regulation - drug effects
Glycerol - metabolism
Insulin - metabolism
Insulin Secretion
Insulin-Secreting Cells - drug effects
Insulin-Secreting Cells - metabolism
Mercuric Chloride - pharmacology
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sulfadiazine - pharmacology
Time Factors
Urea - pharmacology
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Summary:Both mouse and rat pancreatic islet β‐cells were recently found to express aquaglyceroporin 7 (AQP7). In the present study, the expression and role of AQP7 in the function of BRIN‐BD11 cells were investigated. AQP7 mRNA and protein were detected by RT‐PCR and Western blot analysis, respectively. In an isoosmolar medium, the net uptake of [2‐3H]glycerol displayed an exponential time course reaching an equilibrium plateau value close to its extracellular concentration. Within 2 min of incubation in a hypotonic medium (caused by a 50 mM decrease in NaCl concentration), the [2‐3H]glycerol uptake averaged 143.2 ± 3.8% (n = 24; P 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.21872