Simultaneous Determination of Five Benzimidazoles in Feeds Using High-Performance Capillary Electrophoresis

A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm x 75 μm id fused-silica uncoated capillary and injected by hydrody...

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Bibliographic Details
Published in:Journal of AOAC International 2009-07, Vol.92 (4), p.1009-1015
Main Authors: Shen, Jianzhong, Tong, Jing, Jiang, Haiyang, Rao, Qinxiong, Li, Na, Guo, Liming, Ding, Shuangyang
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animal feeding and feeds
anthelmintics
benzimidazole fungicides
benzimidazoles
Benzimidazoles - analysis
Biological and medical sciences
Buffers
capillary zone electrophoresis
chemical structure
Chromatography, High Pressure Liquid
Electrophoresis, Capillary
Feed and pet food industries
feed composition
feeds
Food industries
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
Hydrogen-Ion Concentration
Indicators and Reagents
Mebendazole
medicated feeds
Organic acids
Pharmaceutical Preparations - analysis
quantitative analysis
Reference Standards
veterinary drugs
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Summary:A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm x 75 μm id fused-silica uncoated capillary and injected by hydrodynamic injection (35 mbar x 8 s) at a wavelength detection of 295 nm. The drugs were completely resolved in 50 mmol/L citric acid buffer (pH 1.9) with 30 kV at a temperature of 25°C. Linear range was 1-80 μg/mL; limits of detection for the analytes were less than 2 mg/kg; the overall recoveries for benzimidazoles were >73%, with coefficients of variation below 10%.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/92.4.1009