p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication

BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 p...

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Bibliographic Details
Published in:Journal of virological methods 2009-11, Vol.161 (2), p.308-311
Main Authors: Capocefalo, A, Franceschi, V, Whitelaw, C B A, Vasey, D B, Lillico, S G, Cavirani, S, Donofrio, G
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Cattle
Cattle Diseases - metabolism
Cattle Diseases - virology
Cell Line
Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis
Genes, Reporter
Herpesviridae Infections - metabolism
Herpesviridae Infections - veterinary
Herpesviridae Infections - virology
Herpesvirus 4, Bovine - physiology
Host-Pathogen Interactions
Mice
Promoter Regions, Genetic
Time Factors
Tumor Virus Infections - metabolism
Tumor Virus Infections - veterinary
Tumor Virus Infections - virology
Virus Replication
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Summary:BoHV-4 replication cycle is dependent on the S-phase of the cell-cycle at the stage of viral DNA synthesis. Because p21 is a rate-limiting regulator of the G1/S-phase transition and up-regulated by DNA-damaging agents, in this study p21 expression in BoHV-4 infected cells was investigated. The p21 promoter was found to be highly activated in a dose- and time-dependent manner following BoHV-4 infection only in cells which are permissive for BoHV-4 replication. Thus p21 expression reports on BoHV-4 replication and could represent a host cell defensive response to infection-associated cellular damage.
ISSN:1879-0984
DOI:10.1016/j.jviromet.2009.06.012