Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input

In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing p...

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Bibliographic Details
Published in:Journal of virological methods 2009-11, Vol.161 (2), p.280-283
Main Authors: Weissenborn, S.J., Neale, R., de Koning, M.N.C., Waterboer, T., Abeni, D., Bouwes Bavinck, J.N., Wieland, U., Pfister, H.J.
Format: Article
Language:English
Subjects:
Beta papillomavirus
beta-Globins - genetics
Betapapillomavirus - genetics
Betapapillomavirus - isolation & purification
Biological and medical sciences
Cellular DNA
Detection
DNA - genetics
DNA - isolation & purification
Epidemiology
Eyebrows - physiology
Eyebrows - virology
Female
Fundamental and applied biological sciences. Psychology
Hair Follicle - physiology
Hair Follicle - virology
HPV
Human papillomavirus
Humans
Male
Microbiology
Multiplicity
Papillomavirus Infections - epidemiology
Papillomavirus Infections - genetics
Papillomavirus Infections - virology
PCR
Polymerase Chain Reaction - methods
Prevalence
Sensitivity and Specificity
Sex Factors
Techniques used in virology
Virology
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Summary:In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally equal amounts of cellular DNA should be employed. When cellular DNA input varies this should be clearly taken into account in assessing viral prevalence and multiplicity.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2009.06.024