Targeting xa13, a recessive gene for bacterial blight resistance in rice

• Published in: Theoretical and applied genetics 2006-02, Vol.112 (3), p.455-461
• Main Authors: Chu, Z, Fu, B, Yang, H, Xu, C, Li, Z, Sanchez, A, Park, Y.J, Bennetzen, J.L, Zhang, Q, Wang, S
• Format: Article
• Language: English
• Subjects: Bacteria; bacterial diseases of plants; Biological and medical sciences; blight; chromosome mapping; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; cleaved amplification polymorphism sequence; disease resistance; DNA, Plant; Fundamental and applied biological sciences. Psychology; Gene Targeting; Genes, Plant; Genes, Recessive; Genetic Markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genome, Plant; Genomics; microsatellite repeats; Oryza - genetics; Oryza - microbiology; Oryza sativa; Physical Chromosome Mapping; Plant Diseases - genetics; Plant Diseases - microbiology; plant pathogenic bacteria; Polymerase Chain Reaction; Pteridophyta, spermatophyta; R genes; recessive genes; restriction fragment length polymorphism; Rice; Sequence Analysis, DNA; Vegetals; xa13 gene; Xanthomonas - pathogenicity; Xanthomonas oryzae; Xanthomonas oryzae pv. oryzae
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-0145-6

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide. Thirty bacterial blight resistance (R) genes (21 dominant genes and 9 recessive genes) in rice have been identified. They are the main sources for the genetic improvement of rice for resistance to Xoo. However, little is known about the recessive R genes. To clone and characterize the recessive R genes, we fine-mapped xa13, a fully recessive gene for Xoo resistance, to a DNA fragment of 14.8 kb using the map-based cloning strategy and a series of sequence-based molecular markers. Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes (an extensin-like gene and a homologue of nodulin MtN3) and the 5' end of a predicted hypothetical gene. These results will greatly facilitate the isolation and characterization of xa13. Four PCR-based markers, E6a, SR6, ST9 and SR11 that were tightly linked to the xa13 locus, were also developed. These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs.