Transcription and RNA processing during expression of genes preceding DNA ligase gene 30 in T4-related bacteriophages

• Published in: Virology (New York, N.Y.) N.Y.), 2006-01, Vol.344 (2), p.378-390
• Main Authors: Truncaite, Lidija, Zajančkauskaite, Aurelija, Arlauskas, Aivaras, Nivinskas, Rimas
• Format: Article
• Language: English
• Subjects: Bacteriophage T4 - classification; Bacteriophage T4 - genetics; Base Sequence; DNA Ligases - genetics; Early promoters; Escherichia coli; Gene Expression Regulation, Viral; Genes, Viral - genetics; Genomic region 30.9–30.6; Molecular Sequence Data; Promoter Regions, Genetic; RNA Processing, Post-Transcriptional; RNA secondary structures; RNA, Viral - genetics; RNA, Viral - metabolism; RNase E; RNase RegB; T4-type bacteriophages; Transcription, Genetic
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2005.09.001

Early gene expression in bacteriophage T4 is controlled primarily by the unique early promoters, while T4-encoded RegB endoribonuclease promotes degradation of many early messages contributing to the rapid shift of gene expression from the early to middle stages. The regulatory region for the genes clustered upstream of DNA ligase gene 30 of T4 was known to carry two strong early promoters and two putative RegB sites. Here, we present the comparative analysis of the regulatory events in this region of 16 T4-type bacteriophages. The regulatory elements for control of this gene cluster, such as rho-independent terminator, at least one early promoter, the sequence for stem-loop structure, and the RegB cleavage sites have been found to be conserved in the phages studied. Also, we present experimental evidence that the initial cleavage by RegB of phages TuIa and RB69 enables degradation of early phage mRNAs by the major Escherichia coli endoribonuclease, RNase E.