Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering

Here, we present extracellular matrix (ECM) powders derived from human adipose tissue as injectable cell delivery carriers for adipose tissue engineering. We postulate that human adipose tissue may provide an ideal biomaterial because it contains large amounts of ECM components including collagen. F...

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Bibliographic Details
Published in:Journal of controlled release 2009-10, Vol.139 (1), p.2-7
Main Authors: Choi, Ji Suk, Yang, Hyun-Jin, Kim, Beob Soo, Kim, Jae Dong, Kim, Jun Young, Yoo, Bongyoung, Park, Kinam, Lee, Hee Young, Cho, Yong Woo
Format: Article
Language:English
Subjects:
Adipocytes - physiology
Adipose Tissue - chemistry
Adipose-derived stem cell (ASC)
Adult
Animals
Biological and medical sciences
Cell Adhesion
Cell Proliferation
Cell Separation
Drug Carriers
Drug Delivery Systems
Extracellular matrix (ECM)
Extracellular Matrix - chemistry
General pharmacology
Humans
Injectable cell delivery
Medical sciences
Mice
Microscopy, Electron, Scanning
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Powders
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - physiology
Tissue Engineering
Young Adult
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Summary:Here, we present extracellular matrix (ECM) powders derived from human adipose tissue as injectable cell delivery carriers for adipose tissue engineering. We postulate that human adipose tissue may provide an ideal biomaterial because it contains large amounts of ECM components including collagen. Fresh human adipose tissue was obtained by a simple surgical operation (liposuction). After removing blood and oil components, the tissue was homogenized, centrifuged, freeze-dried, and ground to powders by milling. In an in vitro study, the human ECM powders were highly effective for promotion of cell attachment and proliferation for three-dimensional (3D) cell culture. In in vivo studies, suspensions of human ECM powders containing human adipose-derived stem cells (hASCs) were subcutaneously injected into nude mice. At eight weeks post-injection, numerous blood vessels were observed and the newly formed tissue exhibited adipogenesis with accumulated intracellular small lipid droplets. Overall, the grafts showed well-organized adipose tissue constructs without any signs of tissue necrosis, cystic spaces, or fibrosis. We believe that human ECM powders could act as efficient injectable biomaterials for tissue engineering and have great potential for meeting clinical challenges in regenerative medicine, particularly in relation to adipose tissue engineering. [Display omitted]
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2009.05.034