Prevention of thermal induced aggregation of cytochrome c at isoelectric pH values by polyanions

Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4‐styrene‐sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions,...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2006-02, Vol.93 (3), p.485-493
Main Authors: FEDUNOVA, Diana, ANTALIK, Marian
Format: Article
Language:English
Subjects:
aggregation
Biological and medical sciences
Biotechnology
Calorimetry, Differential Scanning
Chemical reactions
Circular Dichroism
cytochrome c
Cytochromes c - chemistry
Fundamental and applied biological sciences. Psychology
Hot Temperature
Hydrogen-Ion Concentration
Metals
microcalorimetry
polyanion
Polymers
Polymers - chemistry
Polyvinyls - chemistry
Spectrophotometry, Ultraviolet
Sulfonic Acids - chemistry
Thermal energy
thermal stability
viscometry
Viscosity
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Summary:Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4‐styrene‐sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions, both PVS and PSS enhance the thermal transition reversibility of cytochrome c by preventing the aggregation of denatured protein molecules. Data indicate that the polyanions are in complex with cytochrome c that is stabilized by synergistic effect of Coulombic and non‐Coulombic interactions. © 2005 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20733