MCL-1ES, a novel variant of MCL-1, associates with MCL-1L and induces mitochondrial cell death

Myeloid cell leukemia-1 (MCL-1L) is a pro-survival member of the BCL-2 family that promotes cell survival. In this study, we identify a new splicing variant of human MCL-1 that encodes MCL-1ES (extra short). Sequence analysis indicates that this variant results from splicing within the first coding...

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Bibliographic Details
Published in:FEBS letters 2009-09, Vol.583 (17), p.2758-2764
Main Authors: Kim, Jae-Hong, Sim, Se-Hoon, Ha, Hye-Jeong, Ko, Jeong-Jae, Lee, Kangseok, Bae, Jeehyeon
Format: Article
Language:English
Subjects:
Alternative Splicing
Apoptosis
Base Sequence
BCL-2 family
Cell Death - physiology
Cell Line
Cloning, Molecular
Humans
MCL-1 extra short
MCL-1L
Mitochondria - metabolism
Molecular Sequence Data
Myeloid Cell Leukemia Sequence 1 Protein
Myeloid cell leukemia-1
Protein Isoforms - genetics
Protein Isoforms - metabolism
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Splicing variant
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Summary:Myeloid cell leukemia-1 (MCL-1L) is a pro-survival member of the BCL-2 family that promotes cell survival. In this study, we identify a new splicing variant of human MCL-1 that encodes MCL-1ES (extra short). Sequence analysis indicates that this variant results from splicing within the first coding exon of MCL-1 at a non-canonical GC–AG donor–acceptor pair. The deduced sequence of MCL-1ES encodes a protein of 197 amino acids, and the PEST (proline, glutamic acid, serine, and threonine) motifs present in MCL-1L are absent. MCL-1ES interacts with MCL-1L and induces mitochondrial cell death, suggesting that alternative splicing of MCL-1 may control the fate of cells. MINT-7255705, MINT-7255718, MINT-7255731, MINT-7255743:MCL1-ES (uniprotkb:Q07820-2) physically interacts (MI:0914) with MCL1-1L (uniprotkb:Q07820-1) by anti tag coimmunoprecipitation (MI:0007) MINT-7255771:MCL1-ES (uniprotkb:Q07820-2) physically interacts (MI:0914) with Beta actin (uniprotkb:P60709) by anti tag coimmunoprecipitation (MI:0007) MINT-7255781:MCL1-ES (uniprotkb:Q07820-2) physically interacts (MI:0914) with GAPDH (uniprotkb:P04406) by anti tag coimmunoprecipitation (MI:0007) MINT-7255756:MCL1-ES (uniprotkb:Q07820-2) physically interacts (MI:0914) with COX IV (uniprotkb:P13073) by anti tag coimmunoprecipitation (MI:0007)
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2009.08.006