Quantifying the l-arginine paradox in vivo

NO and PO 2 microelectrodes were used to quantify the effects of increased availability of l-arginine in an exteriorized rat mesentery and small intestine microcirculatory preparation in n = 16 rats. During short periods of elevated l-arginine added to the superfusion bath, transient changes in peri...

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Bibliographic Details
Published in:Microvascular research 2006, Vol.71 (1), p.48-54
Main Authors: Vukosavljevic, Nina, Jaron, Dov, Barbee, Kenneth A., Buerk, Donald G.
Format: Article
Language:English
Subjects:
Animals
Arginine - pharmacology
Arterioles - metabolism
Biological Availability
Carrier Proteins - blood
eNOS
l-Arginine
Laser Doppler flowmetry
Male
Mesentery - blood supply
Microcirculation - drug effects
Microcirculation - physiopathology
Microelectrodes
Nitric oxide
Nitric Oxide - blood
Nitric Oxide - metabolism
Oxygen - blood
Partial Pressure
Perfusion
PO 2
Rats
Rats, Sprague-Dawley
Regional Blood Flow - drug effects
Time Factors
Venules - metabolism
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Summary:NO and PO 2 microelectrodes were used to quantify the effects of increased availability of l-arginine in an exteriorized rat mesentery and small intestine microcirculatory preparation in n = 16 rats. During short periods of elevated l-arginine added to the superfusion bath, transient changes in perivascular NO or PO 2 were measured at 171 perivascular sites near intestinal arterioles and venules, simultaneously with tissue perfusion using laser Doppler flowmetry (LDF). Excess l-arginine increased perivascular NO over twofold, by 411 ± 42 nM above the baseline of 329 ± 30 nM ( P 
ISSN:0026-2862
1095-9319
DOI:10.1016/j.mvr.2005.10.006