Precise Characterization of Human Histones in the H2A Gene Family by Top Down Mass Spectrometry

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post...

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Bibliographic Details
Published in:Journal of proteome research 2006-02, Vol.5 (2), p.248-253
Main Authors: Boyne, Michael T, Pesavento, James J, Mizzen, Craig A, Kelleher, Neil L
Format: Article
Language:English
Subjects:
Acetylation
Amino Acid Sequence
Chromatography, Affinity
HeLa Cells
Histones - chemistry
Humans
Mass Spectrometry
Molecular Sequence Data
Protein Isoforms - chemistry
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Summary:Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle. Keywords: histone H2A • Fourier transform mass spectrometry (FTMS) • Top Down • post-translational modification (PTM) • SILAC • cell cycle • ubiquitination • electron capture dissociation (ECD) • histone code • chromatin
ISSN:1535-3893
1535-3907
DOI:10.1021/pr050269n