Proteomic Analysis of Serum Associated Fucosylated Glycoproteins in the Development of Primary Hepatocellular Carcinoma

Changes in N-linked glycosylation are known to occur during the development of cancer. For example, increased branching of oligosaccharides has been associated with metastasis and has been correlated to tumor progression in human cancers of the breast, colon and melanomas. Increases in core fucosyla...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteome research 2006-02, Vol.5 (2), p.308-315
Main Authors: Comunale, Mary Ann, Lowman, Melissa, Long, Ronald E, Krakover, Jonathan, Philip, Ramila, Seeholzer, Steven, Evans, Alison A, Hann, Hie-Won L, Block, Timothy M, Mehta, Anand S
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - metabolism
Carcinoma, Hepatocellular - complications
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - virology
Electrophoresis, Gel, Two-Dimensional
Female
Glycoproteins - blood
Glycosylation
Hepatitis B - complications
Humans
Immunoglobulins - blood
Liver Neoplasms - complications
Liver Neoplasms - metabolism
Liver Neoplasms - virology
Male
Middle Aged
Serum - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Changes in N-linked glycosylation are known to occur during the development of cancer. For example, increased branching of oligosaccharides has been associated with metastasis and has been correlated to tumor progression in human cancers of the breast, colon and melanomas. Increases in core fucosylation have also been associated with the development of hepatocellular carcinoma (HCC). Chronic infection with the hepatitis B virus is associated with more than 55% of all cases of hepatocellular carcinoma. We show here that increased levels of core fucosylation can be observed via glycan analysis of total serum and are associated with the development of HCC. In a blinded study, the serum glycoproteins derived from people diagnosed with HBV induced liver cancer were found to possess a dramatically higher level of fucosylation. This change occurs on both immunoglobulin molecules and on other serum glycoproteins. Targeted glycoproteomic analysis was used to identify those glycoproteins that are hyperfucosylated in cancer. In total, 19 proteins were found to be hyperfucosylated in cancer. The potential of these proteins as biomarkers of cancer is discussed. Keywords: hepatocellular carcinoma • fucosylation • glycomics • biomarkers
ISSN:1535-3893
1535-3907
DOI:10.1021/pr050328x