High-performance liquid chromatography of lactose with evaporative light scattering detection, applied to determine fine particle dose of carrier in dry powder inhalation products

A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for iso...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2006-02, Vol.1107 (1), p.204-207
Main Authors: Beilmann, Bianca, Langguth, Peter, Häusler, Heribert, Grass, Peter
Format: Article
Language:English
Subjects:
Administration, Inhalation
Aminopropyl column
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Dry powder inhalation products
Evaporative light scattering detection
General pharmacology
Lactose
Lactose - analysis
Light
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Powders - chemistry
Reproducibility of Results
Scattering, Radiation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD < 10%) at an injection volume of 10 μL is 10 μg/mL. Linearity was obtained in the range of 10–80 μg/mL ( R 2 > 0.99). A relative standard deviation (RSD) of 0.5% ( N = 6) demonstrated good precision of the optimized method.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.12.083