Induction of gene expression in response to globular adiponectin in vascular endothelial cells

Adiponectin is an adipocyte-specific protein that plays an important regulatory role in the development or prevention of diabetes and atherosclerosis. In the present study, we examined the effect of a proteolytic cleavage product of adiponectin, known as globular adiponectin (gAd), on induction of g...

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Bibliographic Details
Published in:Life sciences (1973) 2009-09, Vol.85 (11), p.457-461
Main Authors: Tomizawa, Atsuko, Hattori, Yoshiyuki, Kasai, Kikuo
Format: Article
Language:English
Subjects:
Adiponectin
Adiponectin - administration & dosage
Adiponectin - pharmacology
Blotting, Western
Cells, Cultured
Chemokine CCL2 - biosynthesis
DNA, Complementary - biosynthesis
DNA, Complementary - genetics
Endothelial cells
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Gene expression
Gene Expression - drug effects
Humans
Interleukin-6 - biosynthesis
Interleukin-8 - biosynthesis
Interleukin-8 - genetics
Mitogen-Activated Protein Kinases - biosynthesis
Mitogen-Activated Protein Kinases - genetics
NF-kappa B - biosynthesis
NF-kappa B - genetics
Protein Kinases - biosynthesis
Protein Kinases - genetics
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction - drug effects
Signal Transduction - physiology
Signaling pathway
Umbilical Veins - cytology
Umbilical Veins - drug effects
Vascular Cell Adhesion Molecule-1 - biosynthesis
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Summary:Adiponectin is an adipocyte-specific protein that plays an important regulatory role in the development or prevention of diabetes and atherosclerosis. In the present study, we examined the effect of a proteolytic cleavage product of adiponectin, known as globular adiponectin (gAd), on induction of gene expression and activation of various signaling pathways in vascular endothelial cells. We showed that gAd induces the expression of a number of genes using PCR arrays, including MCP-1, VCAM-1, E-selectin, IL-6, and IL-8, all of which have been previously shown to be associated with adiponectin, as well as SOD2, PAI-1, and CSF2, which is a new finding. We also demonstrated that gAd activates AMPK, Akt, and NF-kB, as well as various MAPKs, including ERK1/2, JNK, and p38MAPK. Upstream regulation of gene expression might involve two or more activated pathways which interact with one another.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2009.07.012