Induction of gene expression in response to globular adiponectin in vascular endothelial cells

• Published in: Life sciences (1973) 2009-09, Vol.85 (11), p.457-461
• Main Authors: Tomizawa, Atsuko, Hattori, Yoshiyuki, Kasai, Kikuo
• Format: Article
• Language: English
• Subjects: Adiponectin; Adiponectin - administration & dosage; Adiponectin - pharmacology; Blotting, Western; Cells, Cultured; Chemokine CCL2 - biosynthesis; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Gene expression; Gene Expression - drug effects; Humans; Interleukin-6 - biosynthesis; Interleukin-8 - biosynthesis; Interleukin-8 - genetics; Mitogen-Activated Protein Kinases - biosynthesis; Mitogen-Activated Protein Kinases - genetics; NF-kappa B - biosynthesis; NF-kappa B - genetics; Protein Kinases - biosynthesis; Protein Kinases - genetics; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction - drug effects; Signal Transduction - physiology; Signaling pathway; Umbilical Veins - cytology; Umbilical Veins - drug effects; Vascular Cell Adhesion Molecule-1 - biosynthesis
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2009.07.012

Adiponectin is an adipocyte-specific protein that plays an important regulatory role in the development or prevention of diabetes and atherosclerosis. In the present study, we examined the effect of a proteolytic cleavage product of adiponectin, known as globular adiponectin (gAd), on induction of gene expression and activation of various signaling pathways in vascular endothelial cells. We showed that gAd induces the expression of a number of genes using PCR arrays, including MCP-1, VCAM-1, E-selectin, IL-6, and IL-8, all of which have been previously shown to be associated with adiponectin, as well as SOD2, PAI-1, and CSF2, which is a new finding. We also demonstrated that gAd activates AMPK, Akt, and NF-kB, as well as various MAPKs, including ERK1/2, JNK, and p38MAPK. Upstream regulation of gene expression might involve two or more activated pathways which interact with one another.