Effect of active immunization against inhibin on hormonal concentrations and semen characteristics in Shiba bucks

• Published in: Theriogenology 2006-03, Vol.65 (4), p.691-702
• Main Authors: Medan, M.S., Watanabe, G., Nagura, Y., Fujita, M., Taya, K.
• Format: Article
• Language: English
• Subjects: Active immunization; Animals; Antibodies - blood; Follicle Stimulating Hormone - blood; FSH; Goats - physiology; Immunization - veterinary; Inhibin; Inhibins - immunology; Kinetics; Luteinizing Hormone - blood; Male; Scrotum - anatomy & histology; Seasons; Semen; Semen - cytology; Shiba bucks; Sperm Count; Sperm Motility; Spermatogenesis; Testosterone - blood
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2005.06.007

Active immunization against inhibin increased ovulation rate in females; in males, the effects of active immunization against inhibin on hormonal concentrations and sperm production need more investigation. To test the hypothesis that active immunization against inhibin increases FSH secretion and sperm output, the present study was undertaken to determine the effects of active immunization against inhibin on hormonal profile and sperm production in Shiba bucks. The bucks were actively immunized against inhibin α-subunit (immunized group, n = 6) or Freund adjuvant (control group, n = 5) four times, at 5-weeks intervals. Blood samples were collected twice-weekly and two successive ejaculates of semen were collected (with an artificial vagina) once-weekly. Plasma concentrations of FSH, LH and testosterone were measured by radioimmunoassay (RIA) and sperm motility characteristics were measured by computer-assisted sperm analysis (CASA). All inhibin-immunized bucks produced antibodies against inhibin. Relative to control bucks, in immunized bucks there were significant increases in plasma FSH concentrations and in sperm concentrations from 5 to 9 weeks and from 8 to 11 weeks, respectively, after primary immunization. However, plasma concentrations of LH and testosterone, semen volume, percentage of motile spermatozoa and motility parameters (straight-line velocity, curvilinear velocity and linearity index) were similar in both groups. In conclusion, active immunization against inhibin α-subunit increased FSH secretions and enhanced sperm production in bucks, whereas LH and testosterone concentrations, semen volume and sperm motility parameters were unaffected. Active immunization against inhibin could be used to improve fertility in Shiba bucks.