Factors and methods of pig oocyte and embryo quality improvement and their application in reproductive biotechnology

SUMMARY Compared to other mammalian species, pig oocytes and embryos are characterized by high lipid contents stored mainly as lipid droplets in the cytoplasm. This fact has a negative influence on manipulations on oocytes and embryos and, in general, biotechnological procedures are much less advanc...

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Bibliographic Details
Published in:Reproductive biology 2009-07, Vol.9 (2), p.97-112
Main Author: Scheuerer, Barbara
Format: Article
Language:English
Subjects:
Animals
biotechnology
Cloning, Organism - methods
Cloning, Organism - veterinary
cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Embryo Culture Techniques - veterinary
Embryo, Mammalian - cytology
Embryo, Mammalian - drug effects
Female
Fertilization in Vitro - methods
Fertilization in Vitro - veterinary
in vitro culture
in vitro fertilization
Liposomes - pharmacology
modification
Obstetrics and Gynecology
oocyte and embryo
Oocytes
Phenazines - pharmacology
pig
Pressure
Sus scrofa
Vitamin E - pharmacology
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Summary:SUMMARY Compared to other mammalian species, pig oocytes and embryos are characterized by high lipid contents stored mainly as lipid droplets in the cytoplasm. This fact has a negative influence on manipulations on oocytes and embryos and, in general, biotechnological procedures are much less advanced in pigs than in cows. This paper discusses current methods for modifying porcine oocytes and embryos using in vitro culture or microsurgical manipulation, chemical agents such as cytochalasin B or D, physical means such as centrifugation or increased pressure and the biotechnological implications of these procedures. The presented methods make it possible to modify the characteristics of oocytes and embryos and thus increase their susceptibility to cryopreservation and cloning.
ISSN:1642-431X
DOI:10.1016/S1642-431X(12)60020-5