Enzyme-linked immunosorbent assay with worm vomit and cercarial secretions of Schistosoma mansoni to detect infections in an endemic focus of Burkina Faso

Cercariae and adult Schistosoma mansoni were used to prepare, respectively, cercarial secretions (CS) and worm vomit (WoV). These were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to test the IgG-reactivity of sera obtained in an S. mansoni-endemic area of Burkina Faso. Among the...

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Bibliographic Details
Published in:Journal of helminthology 2006-03, Vol.80 (1), p.19-23
Main Authors: Bahgat, M., Sorgho, H., Ouédraogo, J.B., Poda, J.N., Sawadogo, L., Ruppel, A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Antigens, Helminth - immunology
Biological and medical sciences
Burkina Faso - epidemiology
Child
Cross-Sectional Studies
Diseases caused by trematodes
Endemic Diseases
Enzyme-Linked Immunosorbent Assay - methods
Feces - parasitology
Female
Helminthic diseases
Humans
Immunoglobulin G - immunology
Infectious diseases
Larva - immunology
Male
Medical sciences
Middle Aged
Parasitic diseases
Population Surveillance - methods
Prevalence
Schistosoma mansoni - immunology
Schistosomiases
Schistosomiasis mansoni - diagnosis
Schistosomiasis mansoni - epidemiology
Schistosomiasis mansoni - immunology
Sensitivity and Specificity
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Summary:Cercariae and adult Schistosoma mansoni were used to prepare, respectively, cercarial secretions (CS) and worm vomit (WoV). These were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to test the IgG-reactivity of sera obtained in an S. mansoni-endemic area of Burkina Faso. Among the egg-excreting individuals (n=240), 94.6% reacted positively with WoV, but only 62.9% with CS, thus suggesting a high diagnostic sensitivity of WoV, but not of CS. Among those individuals without detectable eggs in two Kato-Katz thick smears from different stool specimens (n=215), the respective percentages of positive IgG reactivity were 78.1% and 63.3%. These positive reactions in the absence of detectable eggs are interpreted in terms of limited sensitivity of parasitological stool examinations. Optical density values in ELISA with CS, but not with WoV, correlated negatively with age, which may reflect decreasing exposure to cercariae in older individuals.
ISSN:0022-149X
1475-2697
DOI:10.1079/JOH2005312