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Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine

In vitro studies on the pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. Since many bacterial infections are swine-specific, studies on pathogenic mechanisms require appropriate cell lines of porcine origin. We have characterized the permanent porcine intestinal e...

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Published in:Histochemistry and cell biology 2006-03, Vol.125 (3), p.293-305
Main Authors: Schierack, Peter, Nordhoff, Marcel, Pollmann, Marion, Weyrauch, Karl Dietrich, Amasheh, Salah, Lodemann, Ulrike, Jores, Jörg, Tachu, Babila, Kleta, Sylvia, Blikslager, Anthony, Tedin, Karsten, Wieler, Lothar H
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container_title Histochemistry and cell biology
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creator Schierack, Peter
Nordhoff, Marcel
Pollmann, Marion
Weyrauch, Karl Dietrich
Amasheh, Salah
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Kleta, Sylvia
Blikslager, Anthony
Tedin, Karsten
Wieler, Lothar H
description In vitro studies on the pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. Since many bacterial infections are swine-specific, studies on pathogenic mechanisms require appropriate cell lines of porcine origin. We have characterized the permanent porcine intestinal epithelial cell line, IPEC-J2, using a variety of methods in order to assess the usefulness of this cell line as an in vitro infection model. Electron microscopic analyses and histochemical staining revealed the cells to be enterocyte-like with microvilli, tight junctions and glycocalyx-bound mucin. The functional integrity of monolayers was determined by transepithelial electrical resistance (TEER) measurements. Both commensal bacteria and important bacterial pathogens were chosen for study based on their principally different infection mechanisms: obligate extracellular Escherichia coli, facultative intracellular Salmonella and obligate intracellular Chlamydia. We determined the colonization and proliferation of the bacteria on and within the host cells and monitored the host cell response. We verified the expression of mRNAs encoding the cytokines IL-1alpha, -6, -7, -8, -18, TNF-alpha and GM-CSF, but not TGF-beta or MCP-1. IL-8 protein expression was enhanced by Salmonella invasion. We conclude that the IPEC-J2 cell line provides a relevant in vitro model system for porcine intestinal pathogen-host cell interactions.
doi_str_mv 10.1007/s00418-005-0067-z
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subjects Animals
Bacteria - pathogenicity
Bacterial Infections - etiology
Bacterial Infections - microbiology
Bacterial Infections - veterinary
Base Sequence
Cell Line
Cytokines - genetics
DNA Primers - genetics
Epithelial Cells - microbiology
Escherichia coli Infections - etiology
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
In Vitro Techniques
Intestines - cytology
Intestines - microbiology
Microscopy, Electron
Microscopy, Fluorescence
Models, Biological
Reverse Transcriptase Polymerase Chain Reaction
Salmonella Infections, Animal - etiology
Salmonella Infections, Animal - microbiology
Swine - microbiology
Swine Diseases - etiology
Swine Diseases - microbiology
title Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine
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