Integrated microfluidic systems for high-performance genetic analysis

Driven by the ambitious goals of genome-related research, fully integrated microfluidic systems have developed rapidly to advance biomolecular and, in particular, genetic analysis. To produce a microsystem with high performance, several key elements must be strategically chosen, including device mat...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2009-10, Vol.27 (10), p.572-581
Main Authors: Liu, Peng, Mathies, Richard A
Format: Article
Language:English
Subjects:
Automation
Biological and medical sciences
Biotechnology
Communicable Diseases - diagnosis
Deoxyribonucleic acid
Diverse techniques
DNA
DNA - genetics
DNA Fingerprinting - methods
Forensic Genetics - methods
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling - methods
Genetic engineering
Genomes
Heating
Humans
Internal Medicine
Medical research
Microfluidics - methods
Molecular and cellular biology
Molecular Biology - methods
Pathogens
Robotics
Sequence Analysis, DNA - methods
Technological change
Temperature control
Thermal cycling
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Summary:Driven by the ambitious goals of genome-related research, fully integrated microfluidic systems have developed rapidly to advance biomolecular and, in particular, genetic analysis. To produce a microsystem with high performance, several key elements must be strategically chosen, including device materials, temperature control, microfluidic control, and sample/product transport integration. We review several significant examples of microfluidic integration in DNA sequencing, gene expression analysis, pathogen detection, and forensic short tandem repeat typing. The advantages of high speed, increased sensitivity, and enhanced reliability enable these integrated microsystems to address bioanalytical challenges such as single-copy DNA sequencing, single-cell gene expression analysis, pathogen detection, and forensic identification of humans in formats that enable large-scale and point-of-analysis applications.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2009.07.002