novel guanylyl cyclase receptor, BdmGC-1, is highly expressed during the development of the oriental fruit fly Bactrocera dorsalis (Hendel)

• Published in: Insect molecular biology 2006-02, Vol.15 (1), p.69-77
• Main Authors: Chang, J.-C, Yang, R.-B, Chen, Y.-H, Lu, K.-H
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Bactrocera dorsalis; Cell Line; cGMP; complementary DNA; developmental gene expression; developmental stages; enzyme activity; gene expression; Gene Expression Regulation, Developmental; glycoprotein; glycoproteins; Glycosylation; guanylate cyclase; guanylyl cyclase; Humans; insect development; Life Cycle Stages; Membrane Glycoproteins - analysis; messenger RNA; Molecular Sequence Data; nucleotide sequences; Octoxynol; receptor; receptor domains; receptors; Receptors, Guanylate Cyclase-Coupled - genetics; Receptors, Guanylate Cyclase-Coupled - metabolism; RNA, Messenger; sequence analysis; Sequence Analysis, DNA; Tephritidae; Tephritidae - genetics; Tephritidae - growth & development
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/j.1365-2583.2006.00609.x

A novel receptor guanylyl cyclase (GC) has been identified from the oriental fruit fly Bactrocera dorsalis (Hendel) and has been designated BdmGC-1. Protein domain analysis revealed that BdmGC-1 possesses a characteristic domain organization similar to all known receptor GCs but with a unique carboxyl-terminal extension. When overexpressed in 293T cells, BdmGC-1 manifests as a cell-surface glycoprotein with a marked cGMP-generating activity but is unresponsive to all ligands known to activate mammalian receptor GCs. BdmGC-1 mRNAs were highly expressed during development but had low or no expression in adult tissues. On the basis of its unique sequence and distinct developmental expression pattern, BdmGC-1 represents a novel receptor GC that may play a critical role during the development of B. dorsalis.