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Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections
Abstract Because urinary tract infections (UTIs) are a quite common disease, the gold standard for diagnosing UTIs is still bacterial culture, although a large percentage of samples are negative: unnecessary cultures can be reduced by means of an effective screening test. The analytic performance of...
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Published in: | Diagnostic microbiology and infectious disease 2009-10, Vol.65 (2), p.103-107 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract Because urinary tract infections (UTIs) are a quite common disease, the gold standard for diagnosing UTIs is still bacterial culture, although a large percentage of samples are negative: unnecessary cultures can be reduced by means of an effective screening test. The analytic performance of a new urine cytometer, the UF-1000i, has been tested on 1463 urine samples submitted to our laboratory for culture. Bacteria and leukocyte counts have been compared by means of the UF-1000i with colony-forming unit (CFU) quantification on citrate lactose electrolytes deficient agar to assess the best cutoff values. By using quantitative cultures and considering as positive a sample with 10 × 105 CFU/mL, 546 positive samples (37%) were observed. If compared with 10 × 105 CFU/mL, the cutoff values obtained were 125 bacteria/μL and 40 leukocytes/ μL, respectively. Analytic parameters such as sensitivity, specificity, positive predictive value, negative predictive value, and correctly classified incidence were satisfactory. Based on the results obtained in this study, when using the UF-1000i analyzer for a screening test for UTI, a cutoff value of 40 white blood cells/μL should be adopted. The cutoff value for bacteria should be 125/μL for those clinical conditions in which 10 × 105 CFU/mL indicates a positivity. |
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ISSN: | 0732-8893 1879-0070 |
DOI: | 10.1016/j.diagmicrobio.2009.06.003 |