Immunohistochemical expression of ubiquitin and telomerase in cervical cancer

• Published in: Virchows Archiv : an international journal of pathology 2009-09, Vol.455 (3), p.235-243
• Main Authors: de Méndez Morelva, Toro, Antonio, Llombart Bosch
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biodegradation; Biological and medical sciences; Biomarkers, Tumor - analysis; Cervical cancer; Cervix Uteri - chemistry; Female; Female genital diseases; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Original Article; Papillomaviridae - isolation & purification; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Telomerase - analysis; Tissue Array Analysis; Tumors; Ubiquitin - analysis; Uterine Cervical Neoplasms - chemistry; Uterine Cervical Neoplasms - virology
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-009-0818-7

Ubiquitin and telomerase immunohistochemical expression patterns in cervical cancer were compared with normal cervical tissue samples. Eighty-one cervical cancer cases and 22 normal exo–endocervical tissue were examined with polyclonal antibody for ubiquitin and 44G12 clone for telomerase using tissue microarrays. The results were interpreted using a semiquantitative scale The average age of patients was 50.67 years. The most frequent histological types were moderately differentiated epidermoid carcinoma (43.5%), according to the degree of differentiation, and endocervical adenocarcinoma (42.1%). Immunohistochemical findings were as follows: 98.7% of cervical cancers showed immunoexpression for ubiquitin and 52.6% for telomerase. Statistically significant differences were found in tumor immunoreactivity when compared with control tissue ( p