Upregulation of carp GDNF mRNA by the immunomodulator AS101

Although the glia derived neurotrophic factor (GDNF) is defined as a molecule that maintains neuronal cells, it possesses a range of functions outside the nervous system. For example, it is essential for uretric branching in kidney morphogenesis and for regulating the differentiation of stem cells d...

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Bibliographic Details
Published in:Developmental & Comparative Immunology 2006, Vol.30 (5), p.441-446
Main Authors: Okun, Eitan, Saida, Hagar, Albeck, Michael, Sredni, Benjamin, Avtalion, Ramy R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
AS101
Blotting, Northern
Carp
Carps - metabolism
Cells, Cultured
Cloning, Molecular
Cyprinidae
Ethylenes - pharmacology
Fish
GDNF
Glial Cell Line-Derived Neurotrophic Factor - biosynthesis
IL-10
Immunologic Factors - pharmacology
Interleukin-10 - antagonists & inhibitors
Interleukin-10 - metabolism
Kidney - cytology
Kidney - drug effects
Kidney - metabolism
Leukocytes - drug effects
Leukocytes - metabolism
Molecular Sequence Data
PBL
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Up-Regulation
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Summary:Although the glia derived neurotrophic factor (GDNF) is defined as a molecule that maintains neuronal cells, it possesses a range of functions outside the nervous system. For example, it is essential for uretric branching in kidney morphogenesis and for regulating the differentiation of stem cells during spermatogenesis, cardiac, hair follicle and vascular differentiation and the maintenance of immune cells. In the present work, the presence of GDNF in carp peripheral blood leukocytes (PBL) and head kidney cells (HK) was evidenced and its evolutionary importance in both neural and immune systems development was suggested. Using the northern-blot technique, we could observe the expression of two different transcripts of this gene. GDNF upregulation was detected using semi-quantitative PCR, following ex vivo treatment of PBL and HK cells with the immunomodulator AS101 which was previously shown to inhibit IL-10 and to up-regulate GDNF protein levels in human SVG astrocyte cell line, in 6-OHDA hemi-parkinsonian mice in vivo and in rat glomerular mesengial cells in vitro.
ISSN:0145-305X
1879-0089
1365-2567
DOI:10.1016/j.dci.2005.07.002