Eu³⁺-doped Gd₂O₃ nanoparticles as reporters for optical detection and visualization of antibodies patterned by microcontact printing

Lanthanide oxide nanoparticles are promising luminescent probes in bioanalysis, because of their unique spectral properties, photostability, and low-cost synthesis. We report for the first time the application of europium-doped gadolinium oxide (Eu:Gd₂O₃) nanoparticles to the optical imaging of anti...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2006-02, Vol.384 (3), p.631-637
Main Authors: Nichkova, Mikaela, Dosev, Dosi, Perron, Richard, Gee, Shirley J, Hammock, Bruce D, Kennedy, Ian M
Format: Article
Language:English
Subjects:
Adsorption
Antibodies - analysis
Antibodies - chemistry
Europium - chemistry
fluorescence
Gadolinium - chemistry
immunoassays
Lanthanide oxide
Microarray
Microcontact printing
Microscopy, Electron, Scanning - methods
Microscopy, Fluorescence - methods
nanoparticles
Nanoparticles - chemistry
Nanotechnology - methods
Optics and Photonics
Sensitivity and Specificity
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lanthanide oxide nanoparticles are promising luminescent probes in bioanalysis, because of their unique spectral properties, photostability, and low-cost synthesis. We report for the first time the application of europium-doped gadolinium oxide (Eu:Gd₂O₃) nanoparticles to the optical imaging of antibody micropatterns. The nanoparticles were synthesized by spray pyrolysis and coated with antibody (IgG) molecules by physical adsorption. Our experiments showed that the Eu:Gd₂O₃ is a good biocompatible solid support for antibody immobilization. The antibodies (anti-rabbit IgG) immobilized on the nanoparticles had excellent biological activity in the specific recognition reaction with rabbit IgG patterned in line strips (10 μmx10 μm) on a glass substrate by use of a micro-contact printing technique. The specific immunoreaction was confirmed by two independent microscopic techniques--fluorescence and scanning electron microscopy (SEM). Both microscopic images revealed that the nanoparticles were organized into designated structures as defined by the microcontact printing process with negligible non-specific binding. The nanoparticles can be used as fluorescent markers in a variety of immunosensing applications in a microscale format.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-005-0246-8