Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream ( Sparus aurata, L.) using an immunoblot technique

An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream ( Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cel...

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Bibliographic Details
Published in:Veterinary microbiology 2006-03, Vol.113 (1), p.137-141
Main Authors: Cano, I., Alonso, M.C., Garcia-Rosado, E., Saint-Jean, S. Rodriguez, Castro, D., Borrego, Juan J.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - immunology
Aquaculture - methods
Asymptomatic carriers
Biological and medical sciences
Blotting, Western - methods
Cell Line
DNA Virus Infections - diagnosis
DNA Virus Infections - veterinary
DNA Virus Infections - virology
Fish Diseases - diagnosis
Fish Diseases - virology
Fundamental and applied biological sciences. Psychology
Gilt-head seabream
Immune Sera - immunology
Immunoblot
Immunoblotting - methods
Immunoblotting - veterinary
Iridoviridae - chemistry
Iridoviridae - immunology
Iridoviridae - isolation & purification
Lymphocystis disease virus
Microbiology
Miscellaneous
Sea Bream - virology
Sensitivity and Specificity
Virology
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Summary:An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream ( Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. The sensitivity of this technique varied between 10 −1 and 10 2 TCID 50. LCDV has also been detected in fish tissues from both, diseased and asymptomatic gilt-head seabream. For the asymptomatic fish detection, a viral amplification step in cell culture and a subsequent viral concentration using polyethylene glycol (PEG) (600 wt) are required. On the contrary, immunoblot allowed the detection of LCDV antigens directly from tissue homogenates of diseased fish. The method described in this study shows higher sensitivity than classical detection techniques based on cell culture inoculation.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2005.10.038