In vitro and in vivo studies on CCR10 regulation by Annexin A1

The mode of action of annexin A1 (ANXA1) is poorly understood. By using rapid subtraction hybridization we studied the effects of human recombinant ANXA1 and the N-terminal ANXA1 peptide on gene expression in a human larynx cell line. Three genes showed strong downregulation after treatment with ANX...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2006-02, Vol.580 (5), p.1431-1438
Main Authors: Rodrigues-Lisoni, Flavia Cristina, Mehemet, Devinder K., Peitl, Paulo, John, Christopher D., Tajara, Eloiza, Buckingham, Julia C., Solito, Egle
Format: Article
Language:English
Subjects:
Animals
Annexin A1
Annexin A1 - deficiency
Annexin A1 - genetics
Annexin A1-null mice
Cell Line
Epithelial cells
Gene expression
Gene Expression Regulation - drug effects
Host defence
Humans
Larynx - cytology
Male
Mice
Mice, Knockout
Peptide Fragments - pharmacology
Receptors, CCR10
Receptors, Chemokine - physiology
Recombinant Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mode of action of annexin A1 (ANXA1) is poorly understood. By using rapid subtraction hybridization we studied the effects of human recombinant ANXA1 and the N-terminal ANXA1 peptide on gene expression in a human larynx cell line. Three genes showed strong downregulation after treatment with ANXA1. In contrast, expression of CCR10, a seven transmembrane G-protein coupled receptor for chemokine CCL27 involved in mucosal immunity, was increased. Moreover the reduction in CCR10 expression induced by ANXA1 gene deletion was rescued by intravenous treatment with low doses of ANXA1. These findings provide new evidence that ANXA1 modulates gene expression.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.01.072