Ligation or cross-linking of CD40 has different effects on AGS gastric cancer cells

A gastric cancer (GC) cell line, AGS, has high-level expression of CD40, a tumor necrosis factor receptor (TNFR) family member. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating...

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Bibliographic Details
Published in:Cellular immunology 2009, Vol.259 (2), p.135-140
Main Authors: Qi, Chun-Jian, Qian, Ke-Qing, Ning, Yong-Ling, Ma, Hong-Bing, Wang, Shi-Zhong, Zhang, Xue-Guang
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - immunology
CD40
CD40 Antigens - immunology
CD40 Antigens - pharmacology
CD40 Ligand - immunology
CD40 Ligand - pharmacology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - immunology
Cell Line, Tumor
Cell Proliferation
Chromones - pharmacology
Flow Cytometry
Gastric cancer
Humans
Indoles - pharmacology
Morpholines - pharmacology
Nuclear Proteins - genetics
Nuclear Proteins - immunology
Protein Kinase Inhibitors - pharmacology
Pyrroles - pharmacology
Recombinant Proteins - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
RNA - chemistry
RNA - genetics
RNA, Small Interfering - genetics
Stomach Neoplasms - immunology
Vascular Endothelial Growth Factor A - immunology
VEGF
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Summary:A gastric cancer (GC) cell line, AGS, has high-level expression of CD40, a tumor necrosis factor receptor (TNFR) family member. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, and cell death. In this research, we studied the effects of different forms of CD40 stimulation on AGS cells by flow cytometry, Western blotting and siRNA transfection. We found that different forms of CD40 stimulation, either recombinant soluble CD40L (sCD40L, ligation) or agonist anti-CD40 antibody (cross-linking), induced different effects in AGS gastric cancer cells, proliferation or apoptosis. We also showed that VEGF provided a significant contribution to sCD40L-induced proliferation, while agonist anti-CD40 antibody induced GADD45 upregulation and promoted apoptosis.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2009.06.007