Complete genome analysis of a rabies virus isolate from Brazilian wild fox

The complete genome sequence of wild-type rabies virus (RABV) isolated from a wild Brazilian hoary fox (Dusicyon sp.), the BR-Pfx1 isolate, was determined and compared with fixed RABV strains. The genome structure and organization of the BR-Pfx1 isolate were composed of 11,924 nt and included the fi...

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Bibliographic Details
Published in:Archives of virology 2009, Vol.154 (9), p.1475-1488
Main Authors: Mochizuki, Nobuyuki, Kobayashi, Yuki, Sato, Go, Itou, Takuya, Gomes, Alberio Antonio Barros, Ito, Fumio Honma, Sakai, Takeo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution
Amino acids
Animals
Antigens, Viral - genetics
Bats
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brazil - epidemiology
Dusicyon
Foxes
Foxes - virology
Fundamental and applied biological sciences. Psychology
Genes
Genes, Viral - genetics
Genome, Viral
Genomes
Glycoproteins
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Molecular Sequence Data
Nucleocapsid Proteins - genetics
Original Article
Polymerase chain reaction
Proteins
Rabies
Rabies - epidemiology
Rabies - veterinary
Rabies - virology
Rabies virus
Rabies virus - genetics
Rabies virus - isolation & purification
Rhabdovirus
RNA polymerase
Sequence Alignment
Veterinary medicine
Viral Proteins - genetics
Virology
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Summary:The complete genome sequence of wild-type rabies virus (RABV) isolated from a wild Brazilian hoary fox (Dusicyon sp.), the BR-Pfx1 isolate, was determined and compared with fixed RABV strains. The genome structure and organization of the BR-Pfx1 isolate were composed of 11,924 nt and included the five standard genes of rhabdoviruses. Sequences of mRNA start and stop signals for transcription were highly conserved among all structural protein genes of the BR-Pfx1 isolate. All amino acid residues in the glycoprotein (G) gene associated with pathogenicity were retained in the BR-Pfx1 isolate, while unique amino acid substitutions were found in antigenic region I of the nucleoprotein gene and III of G. These results suggest that although the standard genome structure and organization of the RABV isolate are common between the BR-Pfx1 isolate and fixed RABV strains, the unique amino acid substitutions in functional sites of the BR-Pfx1 isolate may result in different biological characteristics from fixed RABV strains.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-009-0475-9