Temporally and spectrally resolved sampling imaging with a specially designed streak camera

• Published in: Optics letters 2006-02, Vol.31 (3), p.368-370
• Main Authors: Qu, Junle, Liu, Lixin, Chen, Danni, Lin, Ziyang, Xu, Gaixia, Guo, Baoping, Niu, Hanben
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Equipment Design; Equipment Failure Analysis; Feasibility Studies; Fundamental and applied biological sciences. Psychology; General aspects, investigation technics, apparatus; Microscopy, Confocal - instrumentation; Microscopy, Confocal - methods; Microscopy, Fluorescence, Multiphoton - instrumentation; Microscopy, Fluorescence, Multiphoton - methods; Spectrometry, Fluorescence - instrumentation; Spectrometry, Fluorescence - methods; Tissues, organs and organisms biophysics
• ISSN: 0146-9592; 1539-4794
• DOI: 10.1364/OL.31.000368

We present a novel sampling imaging technique capable of performing simultaneous two-dimensional measurements of the temporal and spectral characteristics of light-emission processes by use of a specially designed streak camera. A proof-of-principle experiment was performed with a homemade multifocal multiphoton fluorescence microscope. The system was calibrated with a Fabry-Perot etalon and a standard fluorophore solution (rhodamine 6G in ethanol) and was shown to have temporal and spectral resolution of 6.5 ps and 3 nm, respectively, as well as high accuracy and reproducibility in lifetime and spectrum measurement. Temporally and spectrally resolved images of 4 x 4 foci on the sample can be obtained with a snapshot.