The surface activity of pulmonary surfactant from diving mammals

• Published in: Respiratory physiology & neurobiology 2006-02, Vol.150 (2), p.220-232
• Main Authors: Miller, Natalie J., Daniels, Christopher B., Schürch, Samuel, Schoel, W. Michael, Orgeig, Sandra
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Anti-adhesive; Biological and medical sciences; Caniformia - physiology; Captive bubble surfactometry; Cattle; Compressibility; Diving - physiology; Fundamental and applied biological sciences. Psychology; Microscopy, Electron, Transmission - methods; Pinniped; Pulmonary Alveoli - metabolism; Pulmonary Alveoli - ultrastructure; Pulmonary Surfactants - metabolism; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics; Sheep; Surface Tension; Vertebrates: respiratory system; Water and amphibous breathing
• ISSN: 1569-9048; 1878-1519
• DOI: 10.1016/j.resp.2005.03.002

Pinnipeds (seals and sea lions) have developed a specialised respiratory system to cope with living in a marine environment. They have a highly reinforced lung that can completely collapse and reinflate during diving without any apparent side effects. These animals may also have a specialised surfactant system to augment the morphological adaptations. The surface activity of surfactant from four species of pinniped (California sea lion, Northern elephant seal, Northern fur seal and Ringed seal) was measured using a captive bubble surfactometer (CBS), and compared to two terrestrial species (sheep and cow). The surfactant of Northern elephant seal, Northern fur seal and Ringed seal was unable to reduce surface tension ( γ) to normal levels after 5 min adsorption (61.2, 36.7, and 46.2 ± 1.7 mN/m, respectively), but California sea lion was able to reach the levels of the cow and sheep (23.4 mN/m for California sea lion, 21.6 ± 0.3 and 23.0 ± 1.5 mN/m for cow and sheep, respectively). All pinnipeds were also unable to obtain the very low γ min achieved by cow (1.4 ± 0.1 mN/m) and sheep (1.5 ± 0.4 mN/m). These results suggest that reducing surface tension to very low values is not the primary function of surfactant in pinnipeds as it is in terrestrial mammals, but that an anti-adhesive surfactant is more important to enable the lungs to reopen following collapse during deep diving.