The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

• Published in: FEBS letters 2006-02, Vol.580 (5), p.1371-1375
• Main Authors: Hoeks, Joris, Hesselink, Matthijs K.C., Sluiter, Wim, Schaart, Gert, Willems, Jodil, Morrisson, Alastair, Clapham, John C., Saris, Wim H.M., Schrauwen, Patrick
• Format: Article
• Language: English
• Subjects: Animals; Carrier Proteins - genetics; Carrier Proteins - physiology; CD36 Antigens - analysis; Dietary Fats - pharmacology; High fat diet; Ion Channels; Lipid Metabolism; Lipid Peroxidation; Lipids - analysis; Lipotoxicity; Mice; Mice, Knockout; Mitochondrial Proteins; Mitochondrion; Muscles - chemistry; Muscles - metabolism; Reactive oxygen species; Succinate Dehydrogenase - metabolism; Triglycerides - analysis; UCP3; Uncoupling Protein 3
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2006.01.059

Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3 −/− mice showed ∼3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were ∼50% lower in UCP3 −/− mice, in comparison to UCP3 +/+ animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3 −/− and UCP3 +/+ mice.