Rewritable DNA Microarrays

Thiol-terminated single-stranded deoxyribonucleic acids (ssDNA) can be immobilized onto pulsed plasma deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily undergo nucleic acid hybridization. Unlike other methods for oligonucleotide attachment to solid surfa...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-02, Vol.128 (7), p.2280-2285
Main Authors: Schofield, W. C. E, McGettrick, J, Bradley, T. J, Badyal, J. P. S, Przyborski, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Disulfides - chemistry
DNA, Single-Stranded - chemistry
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Probes - chemistry
Spectroscopy, Fourier Transform Infrared
Sulfhydryl Compounds - chemistry
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Summary:Thiol-terminated single-stranded deoxyribonucleic acids (ssDNA) can be immobilized onto pulsed plasma deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily undergo nucleic acid hybridization. Unlike other methods for oligonucleotide attachment to solid surfaces, this approach is shown to be independent of substrate material or geometry, and amenable to highly efficient rewriting.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja056367r