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Intestinal Lamina Propria Dendritic Cell Subsets Have Different Origin and Functions
The intestinal immune system discriminates between tolerance toward the commensal microflora and robust responses to pathogens. Maintenance of this critical balance is attributed to mucosal dendritic cells (DCs) residing in organized lymphoid tissue and dispersed in the subepithelial lamina propria....
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Published in: | Immunity (Cambridge, Mass.) Mass.), 2009-09, Vol.31 (3), p.502-512 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The intestinal immune system discriminates between tolerance toward the commensal microflora and robust responses to pathogens. Maintenance of this critical balance is attributed to mucosal dendritic cells (DCs) residing in organized lymphoid tissue and dispersed in the subepithelial lamina propria. In situ parameters of lamina propria DCs (lpDCs) remain poorly understood. Here, we combined conditional cell ablation and precursor-mediated in vivo reconstitution to establish that lpDC subsets have distinct origins and functions. CD103
+ CX
3CR1
− lpDCs arose from macrophage-DC precursors (MDPs) via DC-committed intermediates (pre-cDCs) through a Flt3L growth-factor
-mediated pathway. CD11b
+ CD14
+ CX
3CR1
+ lpDCs were derived from grafted Ly6C
hi but not Ly6C
lo monocytes under the control of GM-CSF. Mice reconstituted exclusively with CX
3CR1
+ lpDCs when challenged in an innate colitis model developed severe intestinal inflammation that was driven by graft-derived TNF-α-secreting CX
3CR1
+ lpDCs. Our results highlight the critical importance of the lpDC subset balance for robust gut homeostasis. |
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ISSN: | 1074-7613 1097-4180 |
DOI: | 10.1016/j.immuni.2009.06.025 |