Isolation and identification of natural endophytic rhizobia from rice (Oryza sativa L.) through rDNA PCR-RFLP and sequence analysis

Three novel endophytic rhizobial strains (RRE3, RRE5, and RRE6) were isolated from naturally growing surface sterilized rice roots. These isolates had the ability to nodulate common bean (Phaseolus vulgaris). Polymerase chain reaction-restriction fragment length polymorphism and sequencing of 16S rD...

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Bibliographic Details
Published in:Current microbiology 2006-02, Vol.52 (2), p.117-122
Main Authors: Singh, Ramesh K, Mishra, Ravi P N, Jaiswal, Hemant K, Kumar, Vinod, Pandey, Shree P, Rao, Sasi B, Annapurna, Kannapali
Format: Article
Language:English
Subjects:
Bacteria
Burkholderia cepacia
Burkholderia cepacia - classification
Burkholderia cepacia - genetics
Burkholderia cepacia - isolation & purification
Microbiology
Molecular Sequence Data
Oryza - growth & development
Oryza - microbiology
Oryza sativa
Phaseolus vulgaris
Plant Roots - microbiology
Polymorphism, Restriction Fragment Length
Rhizobium leguminosarum
Rhizobium leguminosarum - classification
Rhizobium leguminosarum - genetics
Rhizobium leguminosarum - isolation & purification
Rice
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sequence Homology, Nucleic Acid
Species Specificity
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Summary:Three novel endophytic rhizobial strains (RRE3, RRE5, and RRE6) were isolated from naturally growing surface sterilized rice roots. These isolates had the ability to nodulate common bean (Phaseolus vulgaris). Polymerase chain reaction-restriction fragment length polymorphism and sequencing of 16S rDNA of these isolates revealed that RRE3 and RRE5 are phylogenetically very close to Burkholderia cepacia complex, whereas RRE6 has affinity with Rhizobium leguminosarum bv. phaseoli. Plant infection test using gusA reporter gene tagged construct of these isolates indicated that bacterial cells can go inside and colonize the rice root interiors. A significant increase in biomass and grain yield was also recorded in greenhouse-grown rice plants inoculated with these isolates.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-005-0136-5