Ethanol-induced pseudohyphal transition in the cells of Candida tropicalis: participation of phosphoinositide signal transduction

Abstract Ethanol-induced pseudohyphal development in the cells of Candida tropicalis Pk233 was accompanied by the transient accumulation of inositol 1,4,5-trisphosphate (IP3) that occurred at an early growth stage. The concomitant addition of myo-inositol prevented the activation of IP3 accumulation...

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Bibliographic Details
Published in:FEMS yeast research 2006-03, Vol.6 (2), p.177-185
Main Authors: Suzuki, Takahito, Omi, Kazuo, Ito, Takuya, Takata, Toshiya, Kikutsugi, Hideo, Fujimura, Takaji, Kono, Keita, Kamihara, Teijiro
Format: Article
Language:English
Subjects:
Acetic acid
Calcium
Candida tropicalis
Candida tropicalis - cytology
Candida tropicalis - growth & development
Candida tropicalis - metabolism
Culture Media
Enzyme Activation - physiology
Ethanol
Ethanol - pharmacology
Fungal Proteins - metabolism
Growth stage
inositol
Inositol 1,4,5-Trisphosphate - metabolism
Inositol 1,4,5-trisphosphate receptors
Kinases
Phospholipase C
Phospholipases - metabolism
PI signal transduction
Protein kinase C
Pseudohyphae
pseudohyphal transition
Signal transduction
Signal Transduction - drug effects
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Summary:Abstract Ethanol-induced pseudohyphal development in the cells of Candida tropicalis Pk233 was accompanied by the transient accumulation of inositol 1,4,5-trisphosphate (IP3) that occurred at an early growth stage. The concomitant addition of myo-inositol prevented the activation of IP3 accumulation and cancelled pseudohyphal development in the presence of ethanol. The addition of a specific phospholipase C inhibitor, U73 122, inhibited ethanol-induced pseudohyphal transition at the concentrations of subinhibitory levels of cell growth. Pseudohyphal development was also induced by the Ca2+ ionophore, A23 187 in the absence of ethanol. The effect of A23 187 on the development of pseudohyphae was little influenced by myo-inositol, but stimulated by concomitant addition of 12-O-tetradecanoylphorbol 13-acetate. These results suggest that ethanol activated phospholipase C in competition with myo-inositol, and the resulting IP3-Ca2+ and protein kinase C pathways of PI signal transduction may work in pseudohyphal transition.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1574-1364.2005.00020.x