Primary Cilia of Odontoblasts: Possible Role in Molar Morphogenesis

A primary cilium, a sensory organelle present in almost every vertebrate cell, is regularly described in odontoblasts, projecting from the surfaces of the cells. Based on the hypothesis that the primary cilium is crucial both for dentin formation and possibly in tooth pain transmission, we have inve...

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Bibliographic Details
Published in:Journal of dental research 2009-10, Vol.88 (10), p.910-915
Main Authors: Thivichon-Prince, B., Couble, M.L., Giamarchi, A., Delmas, P., Franco, B., Romio, L., Struys, T., Lambrichts, I., Ressnikoff, D., Magloire, H., Bleicher, F.
Format: Article
Language:English
Subjects:
Adolescent
Animals
Calcium Channels - analysis
Calcium Channels - ultrastructure
Cell Movement
Cells, Cultured
Cilia - physiology
Cytoskeletal Proteins - analysis
Dental Pulp - ultrastructure
Dentin - ultrastructure
Dentistry
Group II Chaperonins
Heat-Shock Proteins - analysis
Heat-Shock Proteins - physiology
Humans
Kinesin - analysis
Mice
Mice, Knockout
Molar - growth & development
Molar - ultrastructure
Molecular Chaperones - analysis
Morphogenesis - physiology
Nerve Fibers - ultrastructure
Odontoblasts - physiology
Odontogenesis - physiology
Proteins - analysis
Transcription Factors - analysis
TRPP Cation Channels - analysis
Tubulin - analysis
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Summary:A primary cilium, a sensory organelle present in almost every vertebrate cell, is regularly described in odontoblasts, projecting from the surfaces of the cells. Based on the hypothesis that the primary cilium is crucial both for dentin formation and possibly in tooth pain transmission, we have investigated the expression and localization of the main cilium components and involvement of the OFD1 gene in tooth morphogenesis. Odontoblasts in vitro express tubulin, inversin, rootletin, OFD1, BBS4, BBS6, ALMS1, KIF3A, PC1, and PC2. In vivo, cilia are aligned parallel to the dentin walls, with the top part oriented toward the pulp core. Close relationships between cilium and nerve fibers are evidenced. Calcium channels are concentrated in the vicinity of the basal body. Analysis of these data suggests a putative role of cilia in sensing the microenvironment, probably related to dentin secretion. This hypothesis is enhanced by the huge defects observed on molars from Ofd1 knockout mice, showing undifferentiated dentin-forming cells.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034509345822