IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP

SUMMARY 1 The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem. 2 Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) w...

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Published in:Clinical and experimental pharmacology & physiology 2006-03, Vol.33 (3), p.197-203
Main Authors: Matuszek, Maria A, Gibson, Karen J, Lumbers, Eugenie R, Simonetta, Giuseppe
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Blood Gas Analysis
Blood Pressure - drug effects
blood vessels
Blotting, Western
cortisol
DNA - analysis
DNA - biosynthesis
DNA - genetics
Electrophoresis, Polyacrylamide Gel
Female
fetal development
Gestational Age
Hydrocortisone - pharmacology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - embryology
Muscle, Smooth, Vascular - enzymology
Pregnancy
Proteins - metabolism
Sheep
smooth muscle
vasculature
α-actin
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Summary:SUMMARY 1 The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem. 2 Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7). 3 Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a‐Actin was identified using a monoclonal mouse anti‐a actin antibody and standardized against tissue protein and DNA content. 4 Between 60 and 144 days gestation, there was an exponential increase in the a‐actin content of vascular smooth muscle cells from fetal sheep aorta (P 
ISSN:0305-1870
1440-1681
DOI:10.1111/j.1440-1681.2006.04346.x