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IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP
SUMMARY 1 The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem. 2 Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) w...
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| Published in: | Clinical and experimental pharmacology & physiology 2006-03, Vol.33 (3), p.197-203 |
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| cited_by | cdi_FETCH-LOGICAL-c4556-ae46152837b4cc0dfcc47553d4ce7f805fd1a3f284564c202067fdd8c9bfce133 |
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| cites | cdi_FETCH-LOGICAL-c4556-ae46152837b4cc0dfcc47553d4ce7f805fd1a3f284564c202067fdd8c9bfce133 |
| container_end_page | 203 |
| container_issue | 3 |
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| container_title | Clinical and experimental pharmacology & physiology |
| container_volume | 33 |
| creator | Matuszek, Maria A Gibson, Karen J Lumbers, Eugenie R Simonetta, Giuseppe |
| description | SUMMARY
1
The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem.
2
Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7).
3
Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a‐Actin was identified using a monoclonal mouse anti‐a actin antibody and standardized against tissue protein and DNA content.
4
Between 60 and 144 days gestation, there was an exponential increase in the a‐actin content of vascular smooth muscle cells from fetal sheep aorta (P |
| doi_str_mv | 10.1111/j.1440-1681.2006.04346.x |
| format | article |
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1
The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem.
2
Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7).
3
Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a‐Actin was identified using a monoclonal mouse anti‐a actin antibody and standardized against tissue protein and DNA content.
4
Between 60 and 144 days gestation, there was an exponential increase in the a‐actin content of vascular smooth muscle cells from fetal sheep aorta (P < 0.0001). a‐Actin concentration (densitometry units (U) relative to DNA 260 nm absorbance (Abs)) was significantly (P < 0.05) higher in the aortae of cortisol‐infused (12 601 2499 U/Abs) fetal sheep compared with those that were saline‐infused (4514 670 U/Abs). a‐Actin (relative to DNA absorbance) of carotid and femoral vessels in cortisol‐infused animals (20 659 4812 U/Abs) compared with those that were saline‐infused (14 461 2645 U/Abs) was increased, but the difference was not significant.
5
Therefore, the a‐actin concentration of the vascular smooth muscle of the aorta increases throughout gestation. Cortisol treatment is associated with further increases in a‐actin concentration in the fetal aorta, indicating that the development of large conduit vessels can be altered by this glucocorticoid.</description><identifier>ISSN: 0305-1870</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/j.1440-1681.2006.04346.x</identifier><identifier>PMID: 16487262</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Science Pty</publisher><subject>Actins - metabolism ; Animals ; Blood Gas Analysis ; Blood Pressure - drug effects ; blood vessels ; Blotting, Western ; cortisol ; DNA - analysis ; DNA - biosynthesis ; DNA - genetics ; Electrophoresis, Polyacrylamide Gel ; Female ; fetal development ; Gestational Age ; Hydrocortisone - pharmacology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - embryology ; Muscle, Smooth, Vascular - enzymology ; Pregnancy ; Proteins - metabolism ; Sheep ; smooth muscle ; vasculature ; α-actin</subject><ispartof>Clinical and experimental pharmacology & physiology, 2006-03, Vol.33 (3), p.197-203</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4556-ae46152837b4cc0dfcc47553d4ce7f805fd1a3f284564c202067fdd8c9bfce133</citedby><cites>FETCH-LOGICAL-c4556-ae46152837b4cc0dfcc47553d4ce7f805fd1a3f284564c202067fdd8c9bfce133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16487262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matuszek, Maria A</creatorcontrib><creatorcontrib>Gibson, Karen J</creatorcontrib><creatorcontrib>Lumbers, Eugenie R</creatorcontrib><creatorcontrib>Simonetta, Giuseppe</creatorcontrib><title>IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>SUMMARY
1
The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem.
2
Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7).
3
Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a‐Actin was identified using a monoclonal mouse anti‐a actin antibody and standardized against tissue protein and DNA content.
4
Between 60 and 144 days gestation, there was an exponential increase in the a‐actin content of vascular smooth muscle cells from fetal sheep aorta (P < 0.0001). a‐Actin concentration (densitometry units (U) relative to DNA 260 nm absorbance (Abs)) was significantly (P < 0.05) higher in the aortae of cortisol‐infused (12 601 2499 U/Abs) fetal sheep compared with those that were saline‐infused (4514 670 U/Abs). a‐Actin (relative to DNA absorbance) of carotid and femoral vessels in cortisol‐infused animals (20 659 4812 U/Abs) compared with those that were saline‐infused (14 461 2645 U/Abs) was increased, but the difference was not significant.
5
Therefore, the a‐actin concentration of the vascular smooth muscle of the aorta increases throughout gestation. Cortisol treatment is associated with further increases in a‐actin concentration in the fetal aorta, indicating that the development of large conduit vessels can be altered by this glucocorticoid.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Blood Gas Analysis</subject><subject>Blood Pressure - drug effects</subject><subject>blood vessels</subject><subject>Blotting, Western</subject><subject>cortisol</subject><subject>DNA - analysis</subject><subject>DNA - biosynthesis</subject><subject>DNA - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>fetal development</subject><subject>Gestational Age</subject><subject>Hydrocortisone - pharmacology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - embryology</subject><subject>Muscle, Smooth, Vascular - enzymology</subject><subject>Pregnancy</subject><subject>Proteins - metabolism</subject><subject>Sheep</subject><subject>smooth muscle</subject><subject>vasculature</subject><subject>α-actin</subject><issn>0305-1870</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNkEtOwzAQhi0EouVxBeQVu4Rx_Ei6YBFF6UNKk4qkhZ2VOo7U0lKIW1GOxUU4Ew6tYIs3Hs3839j6EMIEXGLP3dIljIFDREBcD0C4wCgT7v4EdX8Hp6gLFLhDAh866MKYJQBwEPQcdYhgge8Jr4umo_EkjAqc9XGUPRSjPEtwluKvT8d2R6ltpkWcFtiWszCPpkn4gPNxlhVDPJ7mURLjKE6SvOX7cREmOB_G8eQKndXlyujr432JpnYaDZ0kG4yiMHEU41w4pWaCcC-g_pwpBVWtFPM5pxVT2q8D4HVFSlp7AeOCKQ88EH5dVYHqzWulCaWX6Paw97XZvO202cr1wii9WpUverMzUvhC9DgQGwwOQdVsjGl0LV-bxbpsPiQB2SqVS9mak6052SqVP0rl3qI3xzd287Wu_sCjQxu4PwTeFyv98e_FMoonbWV558AvzFbvf_myebb_pz6Xj-lA9gvo9SZPuZzRb82XjGo</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Matuszek, Maria A</creator><creator>Gibson, Karen J</creator><creator>Lumbers, Eugenie R</creator><creator>Simonetta, Giuseppe</creator><general>Blackwell Science Pty</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200603</creationdate><title>IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP</title><author>Matuszek, Maria A ; Gibson, Karen J ; Lumbers, Eugenie R ; Simonetta, Giuseppe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4556-ae46152837b4cc0dfcc47553d4ce7f805fd1a3f284564c202067fdd8c9bfce133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Blood Gas Analysis</topic><topic>Blood Pressure - drug effects</topic><topic>blood vessels</topic><topic>Blotting, Western</topic><topic>cortisol</topic><topic>DNA - analysis</topic><topic>DNA - biosynthesis</topic><topic>DNA - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>fetal development</topic><topic>Gestational Age</topic><topic>Hydrocortisone - pharmacology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - embryology</topic><topic>Muscle, Smooth, Vascular - enzymology</topic><topic>Pregnancy</topic><topic>Proteins - metabolism</topic><topic>Sheep</topic><topic>smooth muscle</topic><topic>vasculature</topic><topic>α-actin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matuszek, Maria A</creatorcontrib><creatorcontrib>Gibson, Karen J</creatorcontrib><creatorcontrib>Lumbers, Eugenie R</creatorcontrib><creatorcontrib>Simonetta, Giuseppe</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matuszek, Maria A</au><au>Gibson, Karen J</au><au>Lumbers, Eugenie R</au><au>Simonetta, Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>2006-03</date><risdate>2006</risdate><volume>33</volume><issue>3</issue><spage>197</spage><epage>203</epage><pages>197-203</pages><issn>0305-1870</issn><eissn>1440-1681</eissn><abstract>SUMMARY
1
The effects of gestation on a‐actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post‐mortem.
2
Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7).
3
Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a‐Actin was identified using a monoclonal mouse anti‐a actin antibody and standardized against tissue protein and DNA content.
4
Between 60 and 144 days gestation, there was an exponential increase in the a‐actin content of vascular smooth muscle cells from fetal sheep aorta (P < 0.0001). a‐Actin concentration (densitometry units (U) relative to DNA 260 nm absorbance (Abs)) was significantly (P < 0.05) higher in the aortae of cortisol‐infused (12 601 2499 U/Abs) fetal sheep compared with those that were saline‐infused (4514 670 U/Abs). a‐Actin (relative to DNA absorbance) of carotid and femoral vessels in cortisol‐infused animals (20 659 4812 U/Abs) compared with those that were saline‐infused (14 461 2645 U/Abs) was increased, but the difference was not significant.
5
Therefore, the a‐actin concentration of the vascular smooth muscle of the aorta increases throughout gestation. Cortisol treatment is associated with further increases in a‐actin concentration in the fetal aorta, indicating that the development of large conduit vessels can be altered by this glucocorticoid.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>16487262</pmid><doi>10.1111/j.1440-1681.2006.04346.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical and experimental pharmacology & physiology, 2006-03, Vol.33 (3), p.197-203 |
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| language | eng |
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| source | EBSCOhost SPORTDiscus with Full Text; Wiley-Blackwell Read & Publish Collection |
| subjects | Actins - metabolism Animals Blood Gas Analysis Blood Pressure - drug effects blood vessels Blotting, Western cortisol DNA - analysis DNA - biosynthesis DNA - genetics Electrophoresis, Polyacrylamide Gel Female fetal development Gestational Age Hydrocortisone - pharmacology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - embryology Muscle, Smooth, Vascular - enzymology Pregnancy Proteins - metabolism Sheep smooth muscle vasculature α-actin |
| title | IMPACT OF CORTISOL ON α-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP |
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