Analysis of microRNA expression profiles during the cell cycle in synchronized HeLa cells

Cell cycle progression is regulated by both transcriptional and post-transcriptional mechanisms. MicroRNAs (miRNAs) emerge as a new class of small non-coding RNA regulators of cell cycle as recent evidence suggests. It is hypothesized that expression of specific miRNAs oscillates orderly along with...

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Published in:BMB reports 2009-09, Vol.42 (9), p.593-598
Main Authors: Zhou, Jue-Yu, Southern Medical University, Guangzhou, P. R. China, Ma, Wen-Li, Southern Medical University, Guangzhou, P. R. China, Liang, Shuang, Southern Medical University, Guangzhou, P. R. China, Zeng, Ye, Southern Medical University, Guangzhou, P. R. China, Shi, Rong, Southern Medical University, Guangzhou, P. R. China, Yu, Hai-Lang, Southern Medical University, Guangzhou, P. R. China, Xiao, Wei-Wei, Southern Medical University, Guangzhou, P. R. China, Zheng, Wen-Ling, Southern China Genomics Research Center, Guangzhou, P. R. China
Format: Article
Language:English
Subjects:
Biomarkers - analysis
CELL CYCLE
CICLO CELULAR
Computational Biology
CYCLE CELLULAIRE
Flow Cytometry
Gene Expression Profiling
HeLa Cells
Humans
Microarray
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Oligonucleotide Array Sequence Analysis
Real-time quantitative RT-PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
SINCRONIZACION
SYNCHRONISATION
SYNCHRONIZATION
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Summary:Cell cycle progression is regulated by both transcriptional and post-transcriptional mechanisms. MicroRNAs (miRNAs) emerge as a new class of small non-coding RNA regulators of cell cycle as recent evidence suggests. It is hypothesized that expression of specific miRNAs oscillates orderly along with cell cycle progression. However, the oscillated expression patterns of many candidate miRNAs have yet to be determined. Here, we describe miRNA expression profiling in double-thymidine synchronized HeLa cells as cell cycle progresses. Twenty-five differentially expressed miRNAs were classified into five groups based on their cell cycle-dependent expression patterns. The cyclic expression of six miRNAs (miR-221, let-7a, miR-21, miR-34a, miR-24, miR-376b) was validated by real-time quantitative RT-PCR (qRT-PCR). These results suggest that specific miRNAs, along with other key factors are required for maintaining and regulating proper cell cycle progression. The study deepens our understanding on cell cycle regulation.
ISSN:1976-6696
DOI:10.5483/bmbrep.2009.42.9.593