A novel method for collecting vaginal pool for fetal lung maturity studies

Objective Fetal lung maturity (FLM) studies using vaginal pool can guide management in near-term gestations with rupture of membranes. Because an adequate sample may be difficult to collect using a syringe, we tested collection using a sterile sponge. Study Design In this prospective study, vaginal...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2009-10, Vol.201 (4), p.408.e1-408.e4
Main Authors: Gleaton, Kenosha D., MD, White, John C., DPM, MS, Koklanaris, Nikki, MD
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Female
fetal lung maturity testing
Fetal Membranes, Premature Rupture - metabolism
Fetal Membranes, Premature Rupture - therapy
Fetal Organ Maturity
Fluorescence Polarization Immunoassay
Gynecology. Andrology. Obstetrics
Humans
Lung - embryology
Medical sciences
Obstetrics and Gynecology
Phosphatidylglycerols - analysis
Pregnancy
premature rupture of membranes
Prospective Studies
Specimen Handling - methods
Syringes
Vagina
vaginal pool
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Summary:Objective Fetal lung maturity (FLM) studies using vaginal pool can guide management in near-term gestations with rupture of membranes. Because an adequate sample may be difficult to collect using a syringe, we tested collection using a sterile sponge. Study Design In this prospective study, vaginal pool was collected via both a syringe and a sponge from each gravida. Study patients experienced rupture of membranes between 34-41 weeks of gestation. Each sample was analyzed using the TDx-FLM II assay and phosphatidylglycerol (PG) testing. Results Fifty patients were enrolled; 44 demonstrated concordant syringe/sponge results. There was 1 instance of mature TDx-FLM testing using a sponge but not a syringe; PG testing in this case was absent in both samples. Using the κ statistic, agreement between the methods was “substantial” for TDx-FLM and “almost perfect” for PG. Conclusion Collecting vaginal pool with a sponge may provide clinically useful information, particularly when PG is present.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2009.06.070