Faecal shedding and serological cross-sectional study of Lawsonia intracellularis in horses in the state of Minas Gerais, Brazil

• Published in: Equine veterinary journal 2009-07, Vol.41 (6), p.593-596
• Main Authors: Guimaraes-Ladeira, C.V, Palhares, M.S, Oliveira, J.S.V, Ramirez, M.A, Guedes, R.M.C
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; bacterial infections; Brazil - epidemiology; Cross-Sectional Studies; Desulfovibrionaceae Infections - epidemiology; Desulfovibrionaceae Infections - microbiology; Desulfovibrionaceae Infections - veterinary; digestive system diseases; disease outbreaks; disease prevalence; disease transmission; epidemiology; Equine; feces; Feces - microbiology; Female; horse; horse diseases; Horse Diseases - epidemiology; Horses; Immunoglobulin G - blood; Lawsonia Bacteria - isolation & purification; Lawsonia intracellularis; Longitudinal Studies; Male; microbial detection; pathogen shedding; PCR; polymerase chain reaction; Polymerase Chain Reaction - veterinary; proliferative enteropathy; serodiagnosis; Seroepidemiologic Studies
• ISSN: 0425-1644; 2042-3306
• DOI: 10.2746/042516409X407639

Summary Reason for performing the study: Proliferative enteropathy, caused by the intracellular bacterium Lawsonia intracellularis, has been described in horses in Australia, the USA, Canada and European countries but has not been reported in Latin America. The prevalence of the disease in horses worldwide is unknown. Objective: To evaluate the presence of subclinical L. intracellularis infection in horses in the state of Minas Gerais, Brazil. Methods: A longitudinal study using serology and PCR for detecting antibodies (IgG) and shedding of L. intracellularis in faecal samples, respectively, was conducted using a total of 223 horses from 14 different horse farms in Minas Gerais, and from the Veterinary School of UFMG equine herds in Minas Gerais. The immunoperoxidase technique in glass slides was used as the serological test. Results: Twenty‐one horse sera had immunoglobulin G titres of 1:60 and were considered positive. The PCR technique in faeces for L. intracellularis DNA identified 7 horses as faecal shedders. Horses shedding the organism appeared healthy, indicating that subclinical infection of L. intracellularis occurred in the horses. Conclusion: Seropositivity and detection of faecal shedding of L. intracellularis indicates the presence of the agent in the equine population in Minas Gerais. Potential relevance: Results of this study should alert clinicians in countries where proliferative enteropthy in horses has not been reported to consider this disease as a possible cause of enteric disease.