Comparison of preservation solutions in an experimental model of organ cooling in kidney transplantation

Background: Viscous preservation solutions such as University of Wisconsin solution (UW) may be less effective at rapid removal of blood from an organ so that cooling takes longer. This study assessed the temperature changes of kidneys flushed with UW and hyperosmolar citrate (HOC). Methods: Porcine...

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Bibliographic Details
Published in:British journal of surgery 2009-10, Vol.96 (10), p.1215-1221
Main Authors: Kay, M. D., Hosgood, S. A., Bagul, A., Nicholson, M. L.
Format: Article
Language:English
Subjects:
Adenosine - pharmacology
Allopurinol - pharmacology
Animals
Biological and medical sciences
Body Temperature
Cold Temperature
General aspects
Glutathione - pharmacology
Hypertonic Solutions - pharmacology
Hypothermia, Induced - methods
Insulin - pharmacology
Kidney - physiology
Kidney Transplantation
Medical sciences
Models, Biological
Organ Preservation Solutions - pharmacology
Raffinose - pharmacology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the urinary system
Swine
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Summary:Background: Viscous preservation solutions such as University of Wisconsin solution (UW) may be less effective at rapid removal of blood from an organ so that cooling takes longer. This study assessed the temperature changes of kidneys flushed with UW and hyperosmolar citrate (HOC). Methods: Porcine kidneys were retrieved and flushed with 500 ml UW or HOC at 4 °C while monitoring kidney temperature at depths of 5 and 20 mm. Renal function was measured on an isolated organ preservation system. Results: The mean(s.d.) rate of temperature fall was slower with UW (at 20 mm: 0·64(0·11) versus 1·01(0·56) °C per min per 100 g; P = 0·016). The perfusion flow rate required to reduce the temperature to less than 10 °C at a depth of 20 mm was lower in the UW group (P = 0·002). Kidneys flushed with HOC gained more weight than those flushed with UW (mean(s.d.) 50(8) versus 7(13) per cent; P = 0·002). Flushing with UW was associated with less histological injury but there were no significant differences in renal function parameters between the groups. Conclusion: UW cooled kidneys more slowly than HOC, but with no adverse effect on renal function. UW resulted in less oedema and histological injury than HOC. Copyright © 2009 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd. Merits evaluation in a clinical setting
ISSN:0007-1323
1365-2168
DOI:10.1002/bjs.6681