Labeling of proteins and oligopeptides with luminescent lanthanide(III) chelates

Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the correspondin...

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Bibliographic Details
Published in:Journal of peptide science 2006-03, Vol.12 (3), p.199-205
Main Authors: Jaakkola, Lassi, Peuralahti, Jari, Hakala, Harri, Mukkala, Veli-matti, Hurskainen, Pertti, Hovinen, Jari
Format: Article
Language:English
Subjects:
Chelating Agents - chemistry
Indicators and Reagents
labeling
lanthanide(III) chelates
Lanthanoid Series Elements - chemistry
Luminescent Agents - chemistry
Molecular Structure
Oligopeptides - chemical synthesis
Oligopeptides - chemistry
Proteins - chemical synthesis
Proteins - chemistry
solid phase
solution phase
Solutions - chemistry
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Description
Summary:Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine‐based europium(III) chelate designed for solution phase protein labeling is demonstrated. Copyright © 2005 European Peptide Society and John Wiley & Sons, Ltd.
ISSN:1075-2617
1099-1387
DOI:10.1002/psc.697