Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs)

The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically an...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-11, Vol.389 (4), p.663-667
Main Authors: Seib, F. Philipp, Prewitz, Marina, Werner, Carsten, Bornhäuser, Martin
Format: Article
Language:English
Subjects:
Adult
Angiogenesis
Bone Marrow - metabolism
Brain - metabolism
Cells, Cultured
Chemokine
Compliance
Elasticity
Female
Glycosan Extracel
Growth factors
Humans
Male
Mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Multipotent Stem Cells - metabolism
Muscles - metabolism
Protein Array Analysis
Proteins - metabolism
Stiffness
Young Adult
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Summary:The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically and biophysically distinct. Here we use well-defined in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2 days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs in vivo.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.09.051