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Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs)
The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically an...
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| Published in: | Biochemical and biophysical research communications 2009-11, Vol.389 (4), p.663-667 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c420t-39b041daa4c92ade4d0bce63eb24d744ca09ce7a01739ccc48d9c20729b92ebc3 |
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| cites | cdi_FETCH-LOGICAL-c420t-39b041daa4c92ade4d0bce63eb24d744ca09ce7a01739ccc48d9c20729b92ebc3 |
| container_end_page | 667 |
| container_issue | 4 |
| container_start_page | 663 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 389 |
| creator | Seib, F. Philipp Prewitz, Marina Werner, Carsten Bornhäuser, Martin |
| description | The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically and biophysically distinct. Here we use well-defined
in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2
days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs
in vivo. |
| doi_str_mv | 10.1016/j.bbrc.2009.09.051 |
| format | article |
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in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2
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in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2
days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs
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| ispartof | Biochemical and biophysical research communications, 2009-11, Vol.389 (4), p.663-667 |
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| language | eng |
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| source | Elsevier |
| subjects | Adult Angiogenesis Bone Marrow - metabolism Brain - metabolism Cells, Cultured Chemokine Compliance Elasticity Female Glycosan Extracel Growth factors Humans Male Mesenchymal stem cells Mesenchymal Stromal Cells - metabolism Multipotent Stem Cells - metabolism Muscles - metabolism Protein Array Analysis Proteins - metabolism Stiffness Young Adult |
| title | Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs) |
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